e coli  (ATCC)

Journal: Journal of Structural Biology: X

Article Title: Salt bridge disruption in colicin Ib channel-forming domain enhances membrane translocation and bactericidal activity

doi: 10.1016/j.yjsbx.2026.100144

Figure Lengend Snippet: E. coli viability after the treatments of full-length ColIb and salt bridge variants using spot assay and CFU analysis. E. coli ATCC8739 treated with full-length WT ColIb or salt bridge variants, in the a, c absence and b, d presence of the iron chelator, 2,2′ bipyridine (100 μM), were 10-fold serially diluted and spotted. The groups treated with the protein buffer were included as a negative control. The bacterial cell viabilities treated with 0.1 nM and 0.01 nM of protein samples are compared in b . Data represent the mean ± S.D. with n = 3 independent experimental replicates. Statistical analyses were assessed using a one-way ANOVA in GraphPad Prism. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001, n.s., not significant.

Article Snippet: Since the above experimental results demonstrated that disrupting the electrostatic interactions in the C-domain leads to a more loosely packed tertiary structure, which may reduce the unfolding energy required for translocation through CirA and facilitate membrane insertion, we sought to characterize the antibacterial activity by monitoring the viability of E. coli ATCC 8739 treated with full-length ColIb and its salt bridge variants ( , ).

Techniques: Spot Test, Negative Control

Journal: Journal of Structural Biology: X

Article Title: Salt bridge disruption in colicin Ib channel-forming domain enhances membrane translocation and bactericidal activity

doi: 10.1016/j.yjsbx.2026.100144

Figure Lengend Snippet: Bactericidal activity of the WT ColIb C-domain and salt bridge variants using the spot-on-lawn antimicrobial assay. Three-fold serially diluted a , b isolated ColIb C-domain and variants, and c , d full-length ColIb were spotted on the lawn of E. coli ATCC8739. The WT ColIb C-domain and variants were purified at pH 6.0 ( a , b left) and pH 4.5 ( a , b right) for spotting. The C-domain-truncated ColIb variant (TR) was pre-spotted on the plate before spotting b the ColIb C-domain and variants, and d full-length ColIb. Refer to Methods for details.

Article Snippet: Since the above experimental results demonstrated that disrupting the electrostatic interactions in the C-domain leads to a more loosely packed tertiary structure, which may reduce the unfolding energy required for translocation through CirA and facilitate membrane insertion, we sought to characterize the antibacterial activity by monitoring the viability of E. coli ATCC 8739 treated with full-length ColIb and its salt bridge variants ( , ).

Techniques: Activity Assay, Isolation, Purification, Variant Assay

Journal: Comparative Immunology Reports

Article Title: The synthetic peptide GATR-3 shows significant antibacterial and biofilm-inhibition activity against shellfish- and oyster-associated bacteria Vibrio vulnificus and Vibrio parahaemolyticus

doi: 10.1016/j.cirep.2025.200266

Figure Lengend Snippet: EC 50 results for Vibrio strains against GATR-3, LL-37-NH 2 , and Mastoparan-AF-NH 2 peptides. Vibrio vulnificus MO6 EC 50 results against A. GATR-3, B. LL-37-NH 2 , and C. Mastoparan-AF-NH 2 peptides. Vibrio vulnificus JY1701 EC 50 results against D. GATR-3, E. LL-37-NH 2 , and F. Mastoparan-AF-NH 2 peptides. Vibrio parahaemolyticus NY477 EC 50 results against G. GATR-3, H. LL-37-NH 2 , and I. Mastoparan-AF-NH 2 peptides. Vibrio parahaemolyticus SAK11 EC 50 results against J. GATR-3, K. LL-37-NH 2 , and L. Mastoparan-AF-NH 2 peptides. Escherichia coli EC 50 results against M. GATR-3, N. LL-37-NH 2 , and O. Mastoparan-AF-NH 2 peptides.

Article Snippet: Against the control strain E. coli ATCC 25922, GATR-3 was extremely potent, with an EC50 of 4.44 × 10−5 μM (1.27 × 10−4 μg/mL), confirming its broad-spectrum and potent efficacy.

Techniques:

Journal: Comparative Immunology Reports

Article Title: The synthetic peptide GATR-3 shows significant antibacterial and biofilm-inhibition activity against shellfish- and oyster-associated bacteria Vibrio vulnificus and Vibrio parahaemolyticus

doi: 10.1016/j.cirep.2025.200266

Figure Lengend Snippet: Biofilm Formation of Vibrio isolates and E. coli . Quantitative comparison of biofilm formation among Vibrio isolates and E. coli after 24 h incubation. Biofilm biomass was determined by crystal violet staining and measurement of OD₆₀₀. V. vulnificus MO6 and V. parahaemolyticus NY477 exhibited the highest biofilm-forming capacities.

Article Snippet: Against the control strain E. coli ATCC 25922, GATR-3 was extremely potent, with an EC50 of 4.44 × 10−5 μM (1.27 × 10−4 μg/mL), confirming its broad-spectrum and potent efficacy.

Techniques: Comparison, Incubation, Staining

Journal: One Health

Article Title: Prevalence and risk factors of antimicrobial resistance in Staphylococcus spp. and Escherichia coli in fruit bats at high-risk human-wildlife interfaces in Bangladesh

doi: 10.1016/j.onehlt.2025.101300

Figure Lengend Snippet: Prevalence of E. coli and Staphylococcus spp. in feces in fruit bat species of Bangladesh ( n = 369). Numbers within the bars represent culture positive isolates of bacteria detected in the respective bat species.

Article Snippet: The results of susceptibility testing were interpreted following the Clinical Laboratory and Standards Institute (CLSI) guideline and the manufacturer's (OXOID, UK) interpretation criteria using the E. coli ATCC 25922 and Staphylococcus aureus ATCC 25923 as a reference strain for quality control ([ ]; Oxoid [ ]) to interpret the zone of inhibition.

Techniques: Bacteria

Journal: One Health

Article Title: Prevalence and risk factors of antimicrobial resistance in Staphylococcus spp. and Escherichia coli in fruit bats at high-risk human-wildlife interfaces in Bangladesh

doi: 10.1016/j.onehlt.2025.101300

Figure Lengend Snippet: AMR pattern of E. coli in bats. Note: AMX = Amoxicillin, AMP = Ampicillin, CFM = Cefixime, CFP=Cefepime, CTX = Cefotaxime, CRO=Ceftriaxone.

Article Snippet: The results of susceptibility testing were interpreted following the Clinical Laboratory and Standards Institute (CLSI) guideline and the manufacturer's (OXOID, UK) interpretation criteria using the E. coli ATCC 25922 and Staphylococcus aureus ATCC 25923 as a reference strain for quality control ([ ]; Oxoid [ ]) to interpret the zone of inhibition.

Techniques: